Document Type : Original Article
Authors
1
National Gene Bank, Agriculture Research Center (ARC), 9 Gamaa St., 12619, Giza, Egypt.
2
Cell Study Research Department, Field Crops Research Institute, Agriculture Research Center, Giza 12619, Egypt
3
Genetics and Cytology Department, Biotechnology Institute, National Research Centre, Egypt
4
Botany and Microbiology Department, Faculty of Science, Alexandria University, Egypt
5
Genetic Resources Research Department, Field Crops Research Institute, Agriculture Research Center, Egypt.
6
Central Laboratory for Design & Statistical Analysis Research, Agricultural Research Center, Giza, Egypt.
7
Biochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt
8
Botany Department. Women's College for Arts, Science and Education. Ain Shams Univ. Egypt
9
Cell Study Research Department, Field Crops Research Institute, Agriculture Research Center, Giza 12619, Egyp
Abstract
Fifteen landraces out of the 18 faba bean accessions comprised the plant materials used in the present study. PCR-based markers, such as CDDP and SCoT, were implicated to determine the relationships and phylogenetic of the faba germplasm. The diversity (h) averaged 0.33 in total, oscillating from 0.17 to 0.38 for SCoT, with an average of 0.31, and from 0.234 to 0.448 for CDDP, with an average of 0354. The Shannon index (I) revealed a grand average of 0.48, with a mean of 0.52 for CDDP, and 0.464 for SCoT. The effective number of alleles (Ne) ranged from 1.506 (SCoT) to 1.63 (CDDP), with a grand average of 1.564. The chemical composition of all the germplasm was determined. The protein content ranged from 23.083 % to 35.857 % in the tested varieties. L245 had the highest protein content (35.857 %), followed by Sakha 4 (33.633 %). Moreover, this difference also appeared in the heatmap as the reason for the emergence of L82, L221, L217, ALB, L224, Sakha 1, L258, and L253 among the L259 accessions and cultivars. Similarly, the Biplot of the PCA-associated loading plot showed that, while the SCoT and CDDP markers Ash %, crud fiber, pod no/node, protein %, pod length and seed no/pod trait were positively correlated with L 239, L 245, L 180, L 258 and L 221, respectively; seed weight per plant; 100 seed weight; end flowering date; first flowering date; and fruiting date were associated with the Nubria 1 cultivar.
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